Hotspot

ndhD

SSC · 116055-117554 · 1500 bp

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Pi 0.00856
MarkerSeek score 42.90
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1500
variable_sites
44
pi
0.00856
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GTTGTGGCGTCTATGGGATT GATACCCTGGGACGGATGTA 50.0 55.0 58.0 58.0 2165-2195 bp 1.0 83.7
ndhD_p2 TTGGAGTTGGGACCCAAAAG GATACCCTGGGACGGATGTA 50.0 55.0 57.9 58.0 2271-2301 bp 1.0 83.2
ndhD_p3 ATGGGGAGCCTATTGGAGTT GATACCCTGGGACGGATGTA 50.0 55.0 58.1 58.0 2283-2313 bp 1.0 83.2
ndhD_p4 GTTGTGGCGTCTATGGGATT CACTCATTGATTCGTCCGGT 50.0 50.0 58.0 58.0 2355-2372 bp 0.615385 64.8
ndhD_p5 TTGGAGTTGGGACCCAAAAG CACTCATTGATTCGTCCGGT 50.0 50.0 57.9 58.0 2461-2478 bp 0.615385 64.4