Hotspot

ndhA

SSC · 120186-122361 · 2176 bp

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Pi 0.0117
MarkerSeek score 51.40
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2176
variable_sites
79
pi
0.0117
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGTTTGAGACAAGTTGTGAAGA TGGGAGAAGTTGATCGTTGA 36.4 45.0 56.1 56.5 2200-2238 bp 1.0 45.0
ndhA_p2 TGTTTGAGACAAGTTGTGAAGA TGGGAGAAGTTGATCGTTGAA 36.4 42.9 56.1 57.2 2200-2238 bp 1.0 45.0
ndhA_p3 TGTTTGAGACAAGTTGTGAAGAA TGGGAGAAGTTGATCGTTGA 34.8 45.0 56.8 56.5 2200-2238 bp 1.0 45.0
ndhA_p4 TTGTTTGAGACAAGTTGTGAAGA TGGGAGAAGTTGATCGTTGA 34.8 45.0 56.8 56.5 2201-2239 bp 1.0 45.0
ndhA_p5 TGTTTGAGACAAGTTGTGAAGA ATGGGAGAAGTTGATCGTTGA 36.4 42.9 56.1 57.0 2201-2239 bp 1.0 45.0