Pi
0.0119
MarkerSeek score
58.10
Primer available
yes
Label
rpl22-rpl2
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl22-rpl2_p1 | GAACGTCCGCGAATTTGATC | CGAGCCCCAATTGGTAGAAA | 50.0 | 50.0 | 57.9 | 57.9 | 608-617 bp | 1.0 | 81.6 |
| rpl22-rpl2_p2 | CTCATAAGAACGTCCGCGAA | CGAGCCCCAATTGGTAGAAA | 50.0 | 50.0 | 58.1 | 57.9 | 615-624 bp | 1.0 | 81.5 |
| rpl22-rpl2_p3 | GAACGTCCGCGAATTTGATC | CACAACCCCTTGGGGTTATC | 50.0 | 55.0 | 57.9 | 58.2 | 582-591 bp | 1.0 | 81.5 |
| rpl22-rpl2_p4 | GAACGTCCGCGAATTTGATC | GATTGTTCGTCGCCGTAGTA | 50.0 | 50.0 | 57.9 | 57.8 | 513-522 bp | 1.0 | 81.5 |
| rpl22-rpl2_p5 | CTCATAAGAACGTCCGCGAA | CACAACCCCTTGGGGTTATC | 50.0 | 55.0 | 58.1 | 58.2 | 589-598 bp | 1.0 | 81.4 |