Hotspot

psbE-petL

LSC · 66146-67074 · 929 bp

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Pi 0.0138
MarkerSeek score 56.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
929
variable_sites
47
pi
0.0138
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCCACTAGTAGAAAACCA 50.0 40.0 57.9 53.9 1076-1088 bp 1.0 50.7
psbE-petL_p2 CAACCCGCAATGAATAGGGA AGCCACTAGTAGAAAACCAA 50.0 40.0 57.9 53.9 1075-1087 bp 1.0 50.7
psbE-petL_p3 ACTGACGAATAACCAACCCG AAGCCACTAGTAGAAAACCA 50.0 40.0 57.9 53.9 1089-1101 bp 1.0 50.3
psbE-petL_p4 ACTGACGAATAACCAACCCG AGCCACTAGTAGAAAACCAA 50.0 40.0 57.9 53.9 1088-1100 bp 1.0 50.3
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC AGCCACTAGTAGAAAACCAA 50.0 40.0 57.8 53.9 998-1010 bp 1.0 50.2