Hotspot

petA-psbJ

LSC · 64316-65363 · 1048 bp

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Pi 0.0157
MarkerSeek score 60.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1048
variable_sites
54
pi
0.0157
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1085-1149 bp 1.0 82.5
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1087-1151 bp 1.0 82.5
petA-psbJ_p3 GAGAAGGTTCAATTGGCCGA CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1084-1148 bp 1.0 82.1
petA-psbJ_p4 TCGAGAAGGTTCAATTGGCC CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1086-1150 bp 1.0 82.1
petA-psbJ_p5 AGAAGGTTCAATTGGCCGAA TCCGGATTAGGTTCATCCCT 45.0 50.0 57.7 57.5 1084-1148 bp 1.0 81.7