Hotspot

trnS-GCU-trnS-CGA

LSC · 8905-9623 · 719 bp

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Pi 0.0151
MarkerSeek score 57.80
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
719
variable_sites
34
pi
0.0151
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 727-787 bp 1.0 68.7
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 726-786 bp 1.0 68.6
trnS-GCU-trnS-CGA_p3 ACGGAAAGAGAGGGATTCGA ACCACTAAACTATACCCGCT 50.0 45.0 58.2 55.6 781-841 bp 1.0 67.2
trnS-GCU-trnS-CGA_p4 AGCAATCCGACGCTTTAGTC ACCACTAAACTATACCCGCT 50.0 45.0 58.4 55.6 729-789 bp 1.0 65.8
trnS-GCU-trnS-CGA_p5 CGACGCTTTAGTCCACTCAG ACCACTAAACTATACCCGCT 55.0 45.0 58.4 55.6 722-782 bp 1.0 65.7