Hotspot

APQ78_gp030-APQ78_gp029

Genome · 62571-63151 · 581 bp

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Pi 0.104
MarkerSeek score 83.10
Primer available yes
Label APQ78_gp030-APQ78_gp029

Feature Metadata

label_name
APQ78_gp030-APQ78_gp029
feature_type
igs
region
Genome
length_bp
581
variable_sites
122
pi
0.104
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.92
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp030-APQ78_gp029_p1 AAGGGACTCTTGGAACACCA CACGATACCAAGGTAAACCCA 50.0 47.6 58.5 57.7 564-702 bp 1.0 77.4
APQ78_gp030-APQ78_gp029_p2 AGGGACTCTTGGAACACCAA CACGATACCAAGGTAAACCCA 50.0 47.6 58.5 57.7 563-701 bp 1.0 77.4
APQ78_gp030-APQ78_gp029_p3 AAGGGACTCTTGGAACACCA ACACGATACCAAGGTAAACCC 50.0 47.6 58.5 57.7 565-703 bp 1.0 77.4
APQ78_gp030-APQ78_gp029_p4 AGGGACTCTTGGAACACCAA ACACGATACCAAGGTAAACCC 50.0 47.6 58.5 57.7 564-702 bp 1.0 77.4
APQ78_gp030-APQ78_gp029_p5 GGGACTCTTGGAACACCAAT CACGATACCAAGGTAAACCCA 50.0 47.6 57.4 57.7 562-700 bp 1.0 76.6