Hotspot

APQ78_gp048-APQ78_gp047

Genome · 46960-47623 · 664 bp

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Pi 0.102
MarkerSeek score 83.60
Primer available yes
Label APQ78_gp048-APQ78_gp047

Feature Metadata

label_name
APQ78_gp048-APQ78_gp047
feature_type
igs
region
Genome
length_bp
664
variable_sites
150
pi
0.102
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp048-APQ78_gp047_p1 CTGACCGTAGACTAGAGCCA GTAAGTCCATCGGTCCACAC 55.0 55.0 58.0 58.0 1558-1596 bp 1.0 91.6
APQ78_gp048-APQ78_gp047_p2 CTGACCGTAGACTAGAGCCA GGGGACGACCATACTTGTTC 55.0 55.0 58.0 58.0 1839-1877 bp 1.0 91.5
APQ78_gp048-APQ78_gp047_p3 CATCCAGTACCGGACCAATG GTAAGTCCATCGGTCCACAC 55.0 55.0 58.0 58.0 941-979 bp 0.833333 84.9
APQ78_gp048-APQ78_gp047_p4 CTGACCGTAGACTAGAGCCA ACGAAACGATCTCTCCAACG 55.0 50.0 58.0 58.0 1990-2028 bp 0.833333 83.8
APQ78_gp048-APQ78_gp047_p5 CGAGTCTCTACAGGGCCTAA GTAAGTCCATCGGTCCACAC 55.0 55.0 57.9 58.0 1261-1262 bp 0.5 60.2