Hotspot

APQ78_gp051-APQ78_gt014

Genome · 41269-41767 · 499 bp

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Pi 0.0953
MarkerSeek score 83.60
Primer available yes
Label APQ78_gp051-APQ78_gt014

Feature Metadata

label_name
APQ78_gp051-APQ78_gt014
feature_type
igs
region
Genome
length_bp
499
variable_sites
104
pi
0.0953
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp051-APQ78_gt014_p1 TCATTGATCTGCGATCGAGG TTTAACCACTCGGCCATCTC 50.0 50.0 57.6 57.6 530-559 bp 1.0 85.4
APQ78_gp051-APQ78_gt014_p2 TCATTGATCTGCGATCGAGG CTTTAACCACTCGGCCATCT 50.0 50.0 57.6 57.6 531-560 bp 1.0 85.3
APQ78_gp051-APQ78_gt014_p3 TCATTGATCTGCGATCGAGG TTAACCACTCGGCCATCTCT 50.0 50.0 57.6 58.4 529-558 bp 1.0 85.1
APQ78_gp051-APQ78_gt014_p4 TCATTGATCTGCGATCGAGG CCTTTAACCACTCGGCCATC 50.0 55.0 57.6 58.6 532-561 bp 1.0 83.6
APQ78_gp051-APQ78_gt014_p5 TCATTGATCTGCGATCGAGG TAACCACTCGGCCATCTCTC 50.0 55.0 57.6 58.9 528-557 bp 1.0 81.5