Hotspot

APQ78_gt010-APQ78_gp057

Genome · 28779-29531 · 753 bp

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Pi 0.1
MarkerSeek score 82.60
Primer available yes
Label APQ78_gt010-APQ78_gp057

Feature Metadata

label_name
APQ78_gt010-APQ78_gp057
feature_type
igs
region
Genome
length_bp
753
variable_sites
154
pi
0.1
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gt010-APQ78_gp057_p1 ATGGGGGCATACTTATCCGA GGAACTGGCTAATCCATGGG 50.0 55.0 58.0 58.0 1535-1558 bp 0.666667 78.3
APQ78_gt010-APQ78_gp057_p2 ATGGGGGCATACTTATCCGA TGAACAGATCGAGCAAGCTC 50.0 50.0 58.0 58.0 1747-1770 bp 0.666667 77.8
APQ78_gt010-APQ78_gp057_p3 ATGGGGGCATACTTATCCGA AGCTTGGGTGGGGTTAAAAG 50.0 50.0 58.0 58.0 2057-2080 bp 0.666667 76.8
APQ78_gt010-APQ78_gp057_p4 ATGGGGGCATACTTATCCGA AGTTTCTTCAGCTTGGGTGG 50.0 50.0 58.0 58.0 2066-2089 bp 0.666667 76.8
APQ78_gt010-APQ78_gp057_p5 ATGGGGGCATACTTATCCGA GAACAGATCGAGCAAGCTCA 50.0 50.0 58.0 58.0 1746-1769 bp 0.5 63.0