Hotspot

APQ78_gt009-APQ78_gt010

Genome · 28183-28706 · 524 bp

Back to Viscum

Pi 0.0805
MarkerSeek score 85.70
Primer available yes
Label APQ78_gt009-APQ78_gt010

Feature Metadata

label_name
APQ78_gt009-APQ78_gt010
feature_type
igs
region
Genome
length_bp
524
variable_sites
92
pi
0.0805
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gt009-APQ78_gt010_p1 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 578-667 bp 1.0 89.7
APQ78_gt009-APQ78_gt010_p2 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 557-646 bp 1.0 89.2
APQ78_gt009-APQ78_gt010_p3 TGTCCTGAACCACTAGACGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.7 58.1 554-643 bp 1.0 89.1
APQ78_gt009-APQ78_gt010_p4 TGTCCTGAACCACTAGACGA CCGATGACTTACGCCTTACC 50.0 55.0 57.7 58.2 575-664 bp 1.0 88.5
APQ78_gt009-APQ78_gt010_p5 TGTCCTGAACCACTAGACGA TTGAACCGATGACTTACGCC 50.0 50.0 57.7 58.3 580-669 bp 1.0 87.5