Hotspot

APQ78_gt004-APQ78_gt005

Genome · 7992-8410 · 419 bp

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Pi 0.257
MarkerSeek score 77.40
Primer available yes
Label APQ78_gt004-APQ78_gt005

Feature Metadata

label_name
APQ78_gt004-APQ78_gt005
feature_type
igs
region
Genome
length_bp
419
variable_sites
197
pi
0.257
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.91
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gt004-APQ78_gt005_p1 GGGATTTGAACCCTCGGTAC GAGTTGGATCTGCAGCTCAA 55.0 50.0 58.0 57.9 1214-2243 bp 1.0 90.9
APQ78_gt004-APQ78_gt005_p2 GGGATTTGAACCCTCGGTAC CCATGAAACAAGTAGCCGGA 55.0 50.0 58.0 57.9 1187-2216 bp 1.0 90.9
APQ78_gt004-APQ78_gt005_p3 GGGATTTGAACCCTCGGTAC AACTCAGGAAGATGAACGCC 55.0 50.0 58.0 57.9 868-1897 bp 0.833333 84.3
APQ78_gt004-APQ78_gt005_p4 CCATCCGGATCAGACCTTTG AACTCAGGAAGATGAACGCC 55.0 50.0 58.0 57.9 1387-1705 bp 0.5 66.4
APQ78_gt004-APQ78_gt005_p5 CCATCCGGATCAGACCTTTG GAGTTGGATCTGCAGCTCAA 55.0 50.0 58.0 57.9 1733-2051 bp 0.5 65.1