Hotspot

APQ78_gp070-APQ78_gt003

Genome · 6044-6816 · 773 bp

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Pi 0.155
MarkerSeek score 81.60
Primer available yes
Label APQ78_gp070-APQ78_gt003

Feature Metadata

label_name
APQ78_gp070-APQ78_gt003
feature_type
igs
region
Genome
length_bp
773
variable_sites
235
pi
0.155
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.92
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp070-APQ78_gt003_p1 GCTAAGAGCACTTCCAGTCC CAAAGGTCTGATCCGGATGG 55.0 55.0 58.0 58.0 1491-1514 bp 0.5 64.7
APQ78_gp070-APQ78_gt003_p2 GCTAAGAGCACTTCCAGTCC TGGTCCTGTTATTCGGAGGT 55.0 50.0 58.0 58.1 903-932 bp 0.5 64.7
APQ78_gp070-APQ78_gt003_p3 GCTAAGAGCACTTCCAGTCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.0 58.1 888-917 bp 0.5 64.6
APQ78_gp070-APQ78_gt003_p4 CGAGCTAAGAGCACTTCCAG CAAAGGTCTGATCCGGATGG 55.0 55.0 58.1 58.0 1494-1517 bp 0.5 64.1
APQ78_gp070-APQ78_gt003_p5 TTTCTACTCGAGCTCCACCA CAAAGGTCTGATCCGGATGG 50.0 55.0 58.1 58.0 1616-1639 bp 0.5 64.1