Hotspot

APQ78_gp066

Genome · 10303-11656 · 1354 bp

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Pi 0.052
MarkerSeek score 83.90
Primer available yes
Label APQ78_gp066

Feature Metadata

label_name
APQ78_gp066
feature_type
gene
region
Genome
length_bp
1354
variable_sites
163
pi
0.052
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp066_p1 ACCTAAAAACGCCTTGCTCA AGGCCAAGCTGTAGAAGGTA 45.0 50.0 58.0 58.0 2269-2308 bp 0.833333 84.8
APQ78_gp066_p2 AACACGACCTAAAAACGCCT AGGCCAAGCTGTAGAAGGTA 45.0 50.0 58.0 58.0 2275-2314 bp 0.833333 84.6
APQ78_gp066_p3 CACCCGCCATTACTTCATCA AGGCCAAGCTGTAGAAGGTA 50.0 50.0 58.0 58.0 1823-2144 bp 0.666667 75.5
APQ78_gp066_p4 ACACGACCTAAAAACGCCTT AGGCCAAGCTGTAGAAGGTA 45.0 50.0 58.0 58.0 2281-2313 bp 0.5 60.7
APQ78_gp066_p5 TAGTATCCGTGGCTACTGCA AGGCCAAGCTGTAGAAGGTA 50.0 50.0 57.9 58.0 2508-2540 bp 0.5 60.5