Hotspot

eboE

LSC · 50291-51502 · 1212 bp

Back to Vischeria

Pi 0.0437

MarkerSeek score 70.00

Primer available yes

Label eboE

Feature Metadata

label_name: eboE
feature_type: gene
region: LSC
length_bp: 1212
variable_sites: 53
pi: 0.0437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
eboE_p1	GCCTTGGCGTAACAGAGTAA	AGAATTCGTCACGGAGAAGC	50.0	50.0	57.9	58.0	1925 bp	1.0	82.5
eboE_p2	GCCTTGGCGTAACAGAGTAA	GGATAGCCGGAATTGCTGAA	50.0	50.0	57.9	58.0	2179 bp	1.0	82.0
eboE_p3	GAAGATGTGACAGCAGGGAG	AGAATTCGTCACGGAGAAGC	55.0	50.0	58.0	58.0	1692 bp	0.5	59.8
eboE_p4	GAAGATGTGACAGCAGGGAG	GGATAGCCGGAATTGCTGAA	55.0	50.0	58.0	58.0	1946 bp	0.5	59.6
eboE_p5	GTGACAGCAGGGAGTAAAGG	AGAATTCGTCACGGAGAAGC	55.0	50.0	57.9	58.0	1686 bp	0.5	59.2