Hotspot

trnG(gcc)-ycf12

LSC · 70405-70467 · 63 bp

Back to Vischeria

Pi 0.0952

MarkerSeek score 74.80

Primer available yes

Label trnG(gcc)-ycf12

Feature Metadata

label_name: trnG(gcc)-ycf12
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 6
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(gcc)-ycf12_p1	CGTTCAAATCGCGTTACTCG	ATCCCTGTTACCTCCGTGAA	50.0	50.0	58.0	58.1	385 bp	1.0	82.0
trnG(gcc)-ycf12_p2	CGTTCAAATCGCGTTACTCG	AATCCCTGTTACCTCCGTGA	50.0	50.0	58.0	58.1	386 bp	1.0	81.9
trnG(gcc)-ycf12_p3	GGGTTTGGGTTGCTAACTCA	AATCCCTGTTACCTCCGTGA	50.0	50.0	58.0	58.1	861-866 bp	1.0	81.6
trnG(gcc)-ycf12_p4	GGGTTTGGGTTGCTAACTCA	ATCCCTGTTACCTCCGTGAA	50.0	50.0	58.0	58.1	860-865 bp	1.0	81.6
trnG(gcc)-ycf12_p5	GGGTTTGGGTTGCTAACTCA	CTGTTACCTCCGTGAAAGGG	50.0	55.0	58.0	57.9	856-861 bp	1.0	81.3