Hotspot

rps6-ccsA

LSC · 64142-64191 · 50 bp

Back to Vischeria

Pi 0.111

MarkerSeek score 76.40

Primer available yes

Label rps6-ccsA

Feature Metadata

label_name: rps6-ccsA
feature_type: igs
region: LSC
length_bp: 50
variable_sites: 5
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps6-ccsA_p1	ACGTGAATGGGGAGGTTCTA	TATTTCTGGAGCGGTTTGGG	50.0	50.0	58.1	57.9	485-490 bp	1.0	81.4
rps6-ccsA_p2	TACGTGAATGGGGAGGTTCT	TATTTCTGGAGCGGTTTGGG	50.0	50.0	58.1	57.9	486-491 bp	1.0	81.4
rps6-ccsA_p3	ACGTGAATGGGGAGGTTCTA	CAGCCTTTTCGTCTTCAGGA	50.0	50.0	58.1	57.8	627-632 bp	1.0	80.5
rps6-ccsA_p4	TACGTGAATGGGGAGGTTCT	CAGCCTTTTCGTCTTCAGGA	50.0	50.0	58.1	57.8	628-633 bp	1.0	80.5
rps6-ccsA_p5	AGGCTTACAAGTTCGTGGTG	TATTTCTGGAGCGGTTTGGG	50.0	50.0	58.1	57.9	831-836 bp	1.0	79.9