Hotspot

trnI(gau)-ssrA

SSC · 36964-36987 · 24 bp

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Pi 0.0833
MarkerSeek score 75.70
Primer available yes
Label trnI(gau)-ssrA

Feature Metadata

label_name
trnI(gau)-ssrA
feature_type
igs
region
SSC
length_bp
24
variable_sites
2
pi
0.0833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI(gau)-ssrA_p1 TCGAACCAGCGATGTTCTTT TTCAACTCCTACCAGCTCCA 45.0 50.0 57.8 58.0 115 bp 1.0 79.9
trnI(gau)-ssrA_p2 TTCGAACCAGCGATGTTCTT TTCAACTCCTACCAGCTCCA 45.0 50.0 57.8 58.0 116 bp 1.0 79.9
trnI(gau)-ssrA_p3 TCGAACCAGCGATGTTCTTT AGGTTCAACTCCTACCAGCT 45.0 50.0 57.8 58.0 118 bp 1.0 79.8
trnI(gau)-ssrA_p4 TTCGAACCAGCGATGTTCTT AGGTTCAACTCCTACCAGCT 45.0 50.0 57.8 58.0 119 bp 1.0 79.8
trnI(gau)-ssrA_p5 CATTAAATGCATCCGGCTGG TTCAACTCCTACCAGCTCCA 50.0 50.0 57.6 58.0 137 bp 1.0 77.6