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trnN(gtt)-clpN

SSC · 33684-33786 · 103 bp

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Pi 0.117

MarkerSeek score 73.00

Primer available yes

Label trnN(gtt)-clpN

Feature Metadata

label_name: trnN(gtt)-clpN
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 12
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(gtt)-clpN_p1	TGTTCTGGCAACTTTGACCA	ACAATCCCTGGCTTTGTACG	45.0	50.0	57.9	58.2	1142-1151 bp	1.0	81.0
trnN(gtt)-clpN_p2	CCGATTGCTCTACCACTGAG	ACAATCCCTGGCTTTGTACG	55.0	50.0	57.8	58.2	692 bp	1.0	80.4
trnN(gtt)-clpN_p3	AAACCTGCGTGTCTAGCTTC	ACAATCCCTGGCTTTGTACG	50.0	50.0	58.2	58.2	1304-1313 bp	1.0	80.1
trnN(gtt)-clpN_p4	AAAAACCTGCGTGTCTAGCT	ACAATCCCTGGCTTTGTACG	45.0	50.0	57.7	58.2	1306-1315 bp	1.0	79.6
trnN(gtt)-clpN_p5	AAAACCTGCGTGTCTAGCTT	ACAATCCCTGGCTTTGTACG	45.0	50.0	57.7	58.2	1305-1314 bp	1.0	79.6