Hotspot

trnF(gaa)-trnC(gca)

SSC · 27485-27494 · 10 bp

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Pi 0.2

MarkerSeek score 78.00

Primer available yes

Label trnF(gaa)-trnC(gca)

Feature Metadata

label_name: trnF(gaa)-trnC(gca)
feature_type: igs
region: SSC
length_bp: 10
variable_sites: 2
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(gaa)-trnC(gca)_p1	GCAATGGACTGAAGATCCGT	CCTTACCACTTGGCGATACC	50.0	55.0	58.0	58.1	83 bp	1.0	81.3
trnF(gaa)-trnC(gca)_p2	GGACTGAAGATCCGTGTGTC	ATAAGTTTAAGGCGGCACCC	55.0	50.0	58.0	58.2	136 bp	1.0	79.2
trnF(gaa)-trnC(gca)_p3	GCAATGGACTGAAGATCCGT	ATAAGTTTAAGGCGGCACCC	50.0	50.0	58.0	58.2	141 bp	1.0	79.0
trnF(gaa)-trnC(gca)_p4	AGCAATGGACTGAAGATCCG	CCTTACCACTTGGCGATACC	50.0	55.0	57.7	58.1	84 bp	1.0	78.9
trnF(gaa)-trnC(gca)_p5	GGACTGAAGATCCGTGTGTC	TTGCAATCCACTGCCTTACC	55.0	50.0	58.0	58.5	91 bp	1.0	78.1