Hotspot

dnaK-trnF(gaa)

SSC · 27294-27411 · 118 bp

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Pi 0.0932

MarkerSeek score 76.80

Primer available yes

Label dnaK-trnF(gaa)

Feature Metadata

label_name: dnaK-trnF(gaa)
feature_type: igs
region: SSC
length_bp: 118
variable_sites: 11
pi: 0.0932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK-trnF(gaa)_p1	TGAAAGATTTAGAAGAAGAATTAGCT	ACCAACTGAGCTATTTGGGC	26.9	50.0	55.0	58.2	182 bp	1.0	44.8
dnaK-trnF(gaa)_p2	TGAAAGATTTAGAAGAAGAATTAGCT	ACGGATCTTCAGTCCATTGC	26.9	50.0	55.0	58.0	205 bp	1.0	44.3
dnaK-trnF(gaa)_p3	TGAAAGATTTAGAAGAAGAATTAGCT	CGGATCTTCAGTCCATTGCT	26.9	50.0	55.0	57.7	204 bp	1.0	44.3
dnaK-trnF(gaa)_p4	TGAAAGATTTAGAAGAAGAATTAGCT	GACACACGGATCTTCAGTCC	26.9	55.0	55.0	58.0	210 bp	1.0	44.2
dnaK-trnF(gaa)_p5	TGAAAGATTTAGAAGAAGAATTAGC	GACACACGGATCTTCAGTCC	28.0	55.0	53.7	58.0	210 bp	1.0	44.2