Pi
0.0042
MarkerSeek score
61.40
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | ATCAAAGCCACCTCTTCTGC | GTTCCTCGGTGGTCATACAA | 50.0 | 50.0 | 58.2 | 57.2 | 203 bp | 1.0 | 75.4 |
| ycf1_p2 | ACGAAAATCCGATTGTTGCG | GTTCCTCGGTGGTCATACAA | 45.0 | 50.0 | 57.8 | 57.2 | 231 bp | 1.0 | 74.5 |
| ycf1_p3 | TTACGTCTTTGAGCACGCAT | GTTCCTCGGTGGTCATACAA | 45.0 | 50.0 | 58.2 | 57.2 | 272 bp | 1.0 | 74.3 |
| ycf1_p4 | TCCCGACGAAAATCCGATTG | GTTCCTCGGTGGTCATACAA | 50.0 | 50.0 | 58.4 | 57.2 | 236 bp | 1.0 | 73.5 |
| ycf1_p5 | CGTCTTTGAGCACGCATAGA | GTTCCTCGGTGGTCATACAA | 50.0 | 50.0 | 58.4 | 57.2 | 269 bp | 1.0 | 73.1 |
| ycf1_p1 | AGAAAAGAAAAAGGGAGGTTTGTG | CCTTCTTCCATAACCCGAGC | 37.5 | 55.0 | 58.0 | 58.0 | 182 bp | 1.0 | 47.9 |
| ycf1_p2 | AAGAAAAAGGGAGGTTTGTG | CCTTCTTCCATAACCCGAGC | 40.0 | 55.0 | 53.9 | 58.0 | 178 bp | 1.0 | 47.1 |
| ycf1_p3 | AGAAAAGAAAAAGGGAGGTTTGTG | GAGCTCGGAGAAGGAAGAGA | 37.5 | 55.0 | 58.0 | 58.2 | 166 bp | 1.0 | 46.4 |
| ycf1_p4 | AAAGAAAAAGGGAGGTTTGTG | CCTTCTTCCATAACCCGAGC | 38.1 | 55.0 | 54.8 | 58.0 | 179 bp | 1.0 | 46.1 |
| ycf1_p5 | AGAAAAGAAAAAGGGAGGTTTGT | CCTTCTTCCATAACCCGAGC | 34.8 | 55.0 | 56.7 | 58.0 | 182 bp | 1.0 | 45.8 |