Hotspot

petA-psbJ

LSC · 108487-109625 · 1139 bp

Back to Viridantha

Pi 0.00702

MarkerSeek score 64.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 12
pi: 0.00702
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.9	58.0	1248-1265 bp	1.0	81.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.9	58.0	1246-1263 bp	1.0	81.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	50.0	55.0	57.9	57.6	1247-1264 bp	1.0	78.1
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.7	58.0	1255-1272 bp	1.0	76.0
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.7	58.0	1253-1270 bp	1.0	76.0