Hotspot

trnM-CAU-atpE

LSC · 97400-97570 · 171 bp

Back to Viridantha

Pi 0.0468
MarkerSeek score 69.80
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
171
variable_sites
12
pi
0.0468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGCAGGAGTCATTGGTTCAA TAGGACACGAGTAGAGGCTG 50.0 55.0 58.1 58.0 246 bp 1.0 82.9
trnM-CAU-atpE_p2 GGCAGGAGTCATTGGTTCAA CTCTCAGACGAGCTAGGACA 50.0 55.0 58.1 58.0 259 bp 1.0 82.7
trnM-CAU-atpE_p3 GGCAGGAGTCATTGGTTCAA GCGAATCTAGCTCTCAGACG 50.0 55.0 58.1 58.0 269 bp 1.0 82.6
trnM-CAU-atpE_p4 GGCAGGAGTCATTGGTTCAA CGAGCTAGGACACGAGTAGA 50.0 55.0 58.1 58.1 251 bp 1.0 82.6
trnM-CAU-atpE_p5 TTGCTTTCATACGGCAGGAG GCGAATCTAGCTCTCAGACG 50.0 55.0 58.3 58.0 281 bp 1.0 81.1