Hotspot

ndhC-trnV-UAC

LSC · 95450-96499 · 1050 bp

Back to Viridantha

Pi 0.00384
MarkerSeek score 62.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1050
variable_sites
6
pi
0.00384
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1178-1187 bp 1.0 79.2
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC GAGCCCAATGCAACAAACAA 55.0 45.0 58.0 57.8 1265-1274 bp 1.0 78.8
ndhC-trnV-UAC_p3 CGGGGCTAAAACTCCTGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1173-1182 bp 1.0 77.9
ndhC-trnV-UAC_p4 CGGGGCTAAAACTCCTGAAA GAGCCCAATGCAACAAACAA 50.0 45.0 57.8 57.8 1260-1269 bp 1.0 77.5
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAACTCCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1177-1186 bp 1.0 76.7