Hotspot

rpoB-trnC-GCA

LSC · 71239-72625 · 1387 bp

Back to Viridantha

Pi 0.0024

MarkerSeek score 60.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1387
variable_sites: 5
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.4	58.1	1447-1462 bp	1.0	76.8
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.6	58.1	1448-1463 bp	1.0	76.6
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1473-1488 bp	1.0	73.6
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	1474-1489 bp	1.0	73.4
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	47.6	50.0	58.1	58.1	1449-1464 bp	1.0	71.7