Hotspot

matK-trnK-UUU

LSC · 47828-48639 · 812 bp

Back to Viridantha

Pi 0.00823

MarkerSeek score 64.80

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 10
pi: 0.00823
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCATGAGCAAACGC	TTTGATAGGGTCGTGCAGTG	50.0	50.0	57.9	57.9	1021-1027 bp	1.0	79.6
matK-trnK-UUU_p2	CCATGATCATGAGCAAACGC	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.9	57.9	954-960 bp	1.0	79.5
matK-trnK-UUU_p3	TAGGAAGTGTTGTTGCCGAG	TTTGATAGGGTCGTGCAGTG	50.0	50.0	57.8	57.9	971-977 bp	1.0	79.3
matK-trnK-UUU_p4	TAGGAAGTGTTGTTGCCGAG	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.8	57.9	904-910 bp	1.0	79.2
matK-trnK-UUU_p5	CCATGATCATGAGCAAACGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	936-942 bp	1.0	79.1