Hotspot

rpl32-trnL-UAG

SSC · 2842-3603 · 762 bp

Back to Viridantha

Pi 0.0035

MarkerSeek score 61.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 762
variable_sites: 4
pi: 0.0035
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	849-852 bp	1.0	76.2
rpl32-trnL-UAG_p2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.5	58.0	850-853 bp	1.0	76.2
rpl32-trnL-UAG_p3	CACCGGGCATTCAAAAAGTT	TTTAGAAGATGCCGCCACTC	45.0	50.0	57.5	58.0	909-912 bp	1.0	76.0
rpl32-trnL-UAG_p4	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	852-855 bp	1.0	75.7
rpl32-trnL-UAG_p5	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	853-856 bp	1.0	75.7