Hotspot

psbA

LSC · 364-1425 · 1062 bp

Back to Viola

Pi 0.00307
MarkerSeek score 35.80
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
19
pi
0.00307
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 CATGGTGGATTCACAACCCA AAAGGGCGGGTTTGGTATTT 50.0 45.0 58.1 58.0 1995-2208 bp 0.97619 87.7
psbA_p2 CATGGTGGATTCACAACCCA AAGGGCGGGTTTGGTATTTT 50.0 45.0 58.1 58.0 1994-2207 bp 0.97619 87.7
psbA_p3 GATCCACTTGGCTACATCCG AAAGGGCGGGTTTGGTATTT 55.0 45.0 58.1 58.0 1968-2181 bp 0.97619 87.3
psbA_p4 GATCCACTTGGCTACATCCG AAGGGCGGGTTTGGTATTTT 55.0 45.0 58.1 58.0 1967-2180 bp 0.97619 87.3
psbA_p5 CATGGTGGATTCACAACCCA AATTCGTGTGCTTGGGAGTT 50.0 45.0 58.1 58.0 1350-1581 bp 1.0 86.3