Hotspot

trnL-UAG-ccsA

SSC · 118497-118647 · 151 bp

Back to Viola

Pi 0.0326
MarkerSeek score 66.10
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
151
variable_sites
33
pi
0.0326
unique_haplotype_count
36
primer_available
yes
alignment_reliability
0.87
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT TGTTCTAAAGCCGAAAATATCA 50.0 31.8 58.0 53.8 218-253 bp 1.0 52.0
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG TGTTCTAAAGCCGAAAATATCA 50.0 31.8 58.0 53.8 217-252 bp 1.0 52.0
trnL-UAG-ccsA_p3 TAGACACGCTGCTCTTAGGA TGTTCTAAAGCCGAAAATATCA 50.0 31.8 57.9 53.8 208-243 bp 1.0 52.0
trnL-UAG-ccsA_p4 GGTAGACACGCTGCTCTTAG TGTTCTAAAGCCGAAAATATCA 55.0 31.8 57.8 53.8 210-245 bp 1.0 52.0
trnL-UAG-ccsA_p5 GTAGACACGCTGCTCTTAGG TGTTCTAAAGCCGAAAATATCA 55.0 31.8 57.8 53.8 209-244 bp 1.0 52.0