Hotspot

ndhF-trnL-UAG

SSC · 116692-118416 · 1725 bp

Back to Viola

Pi 0.0367
MarkerSeek score 74.00
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1725
variable_sites
366
pi
0.0367
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TAGGTCGCGTGAACCAAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1277-2273 bp 1.0 91.5
ndhF-trnL-UAG_p2 TCGCGTGAACCAAAAACCTA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1273-2269 bp 1.0 91.5
ndhF-trnL-UAG_p3 TAGGTCGCGTGAACCAAAAA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1278-2274 bp 1.0 91.5
ndhF-trnL-UAG_p4 TCGCGTGAACCAAAAACCTA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1274-2270 bp 1.0 91.5
ndhF-trnL-UAG_p5 TCGCGTGAACCAAAAACCTA ACTGAGATTGGAGAGCTGGT 45.0 50.0 58.0 58.1 1864-2863 bp 1.0 91.2