Hotspot

clpP-psbB

LSC · 74179-74917 · 739 bp

Back to Viola

Pi 0.0205
MarkerSeek score 69.60
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
739
variable_sites
80
pi
0.0205
unique_haplotype_count
33
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 AACGGACTTTTGGAACACCA CGATACCAAGGCAAACCCAT 45.0 50.0 57.9 58.2 728-856 bp 1.0 89.0
clpP-psbB_p2 ACGGACTTTTGGAACACCAA CGATACCAAGGCAAACCCAT 45.0 50.0 57.9 58.2 727-855 bp 1.0 89.0
clpP-psbB_p3 AACGGACTTTTGGAACACCA CAAACGACCGGGATCATTCA 45.0 50.0 57.9 58.3 765-893 bp 1.0 88.8
clpP-psbB_p4 ACGGACTTTTGGAACACCAA CAAACGACCGGGATCATTCA 45.0 50.0 57.9 58.3 764-892 bp 1.0 88.8
clpP-psbB_p5 AATTTTTATGGCCCCTCCCG CGATACCAAGGCAAACCCAT 50.0 50.0 58.2 58.2 758-830 bp 0.97619 87.4