Hotspot

ycf4-cemA

LSC · 62223-62660 · 438 bp

Back to Viola

Pi 0.0225
MarkerSeek score 67.10
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
438
variable_sites
54
pi
0.0225
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.93
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA CCACCGGGGCAAAAATACTA 45.0 50.0 57.9 57.9 467-530 bp 1.0 90.3
ycf4-cemA_p2 CTCCACGAGAAGTTGAGCAA CCACCGGGGCAAAAATACTA 50.0 50.0 57.8 57.9 512-575 bp 1.0 89.7
ycf4-cemA_p3 TGCGCGTACCAATTGAAGTA GATCCACCGGGGCAAAAATA 45.0 50.0 57.9 58.2 470-533 bp 1.0 89.5
ycf4-cemA_p4 TGCGCGTACCAATTGAAGTA GAAAAAGAGATCCACCGGGG 45.0 55.0 57.9 58.2 478-541 bp 1.0 89.4
ycf4-cemA_p5 CTCCACGAGAAGTTGAGCAA GATCCACCGGGGCAAAAATA 50.0 50.0 57.8 58.2 515-578 bp 1.0 88.9