Hotspot

psbZ-trnG-GCC

LSC · 36845-37873 · 1029 bp

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Pi 0.0343
MarkerSeek score 70.20
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
1029
variable_sites
166
pi
0.0343
unique_haplotype_count
36
primer_available
yes
alignment_reliability
0.85
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TCTGGTAGGTATTCTTAATTCTCT CGTCTTCTCCTTGGCAAAGA 33.3 50.0 54.3 57.8 878-1653 bp 1.0 52.0
psbZ-trnG-GCC_p2 TCTGGTAGGTATTCTTAATTCTCTT CGTCTTCTCCTTGGCAAAGA 32.0 50.0 55.0 57.8 878-1653 bp 1.0 52.0
psbZ-trnG-GCC_p3 TTCTGGTAGGTATTCTTAATTCTCT CGTCTTCTCCTTGGCAAAGA 32.0 50.0 55.0 57.8 879-1654 bp 1.0 52.0
psbZ-trnG-GCC_p4 TCTGGTAGGTATTCTTAATTCTC CGTCTTCTCCTTGGCAAAGA 34.8 50.0 52.8 57.8 878-1653 bp 1.0 52.0
psbZ-trnG-GCC_p5 TCTGGTAGGTATTCTTAATTCTCT CCATTTCCTTGGGCGGATAG 33.3 55.0 54.3 58.4 913-1688 bp 0.97619 51.0