Hotspot

trnS-GCU-trnG-UCC

LSC · 6495-7136 · 642 bp

Back to Viola

Pi 0.0164
MarkerSeek score 67.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
642
variable_sites
75
pi
0.0164
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.94
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGCCGAAGATCCCTTAACTT 50.0 45.0 58.0 56.5 650-756 bp 1.0 80.2
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGCCGAAGATCCCTTAACTT 50.0 45.0 58.0 56.5 649-755 bp 1.0 80.2
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ATGCCGAAGATCCCTTAACT 50.0 45.0 58.0 56.3 651-757 bp 1.0 78.4
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ATGCCGAAGATCCCTTAACT 50.0 45.0 58.0 56.3 650-756 bp 1.0 78.3
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TGCCGAAGATCCCTTAACTTT 50.0 42.9 58.0 57.3 650-756 bp 1.0 78.3