Hotspot

trnK-UUU-trnQ-UUG

LSC · 4380-5083 · 704 bp

Back to Viola

Pi 0.0433
MarkerSeek score 77.40
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
704
variable_sites
132
pi
0.0433
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.85
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 CGTTAGCCGCACTTAAAAGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 616-787 bp 1.0 90.4
trnK-UUU-trnQ-UUG_p2 GTTAGCCGCACTTAAAAGCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 615-786 bp 1.0 89.3
trnK-UUU-trnQ-UUG_p3 CCGCACTTAAAAGCCGAGTA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 610-781 bp 1.0 89.1
trnK-UUU-trnQ-UUG_p4 AAAAGACGTTAGCCGCACTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 622-793 bp 1.0 88.1
trnK-UUU-trnQ-UUG_p5 AGACGTTAGCCGCACTTAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 619-790 bp 1.0 87.8