Hotspot

ndhG

SSC · 123571-124101 · 531 bp

Back to Vincetoxicum

Pi 0.0728

MarkerSeek score 69.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 129
pi: 0.0728
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CTAATACATCGGTGGGGCAG	TCGCACAATTTCGAATTCGC	55.0	45.0	57.8	58.1	1796-1859 bp	0.833333	75.3
ndhG_p2	TCTAATACATCGGTGGGGCA	TCGCACAATTTCGAATTCGC	50.0	45.0	58.2	58.1	1797-1860 bp	0.833333	75.0
ndhG_p3	TGAAACAATAGCCAGCCCAA	TCGCACAATTTCGAATTCGC	45.0	45.0	57.7	58.1	1411-1474 bp	0.833333	75.0
ndhG_p4	CTAATACATCGGTGGGGCAG	GCACAATTTCGAATTCGCCT	55.0	45.0	57.8	57.7	1794-1857 bp	0.833333	74.2
ndhG_p5	TCTAATACATCGGTGGGGCA	GCACAATTTCGAATTCGCCT	50.0	45.0	58.2	57.7	1795-1858 bp	0.833333	73.9