Hotspot

ndhF

SSC · 115589-117808 · 2220 bp

Back to Vincetoxicum

Pi 0.126

MarkerSeek score 82.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 916
pi: 0.126
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGCTTGGAGCACTAGTACT	TGGGGTCGGTTTAATTTGCA	50.0	45.0	58.1	58.0	2907-2991 bp	0.833333	76.5
ndhF_p2	AGCCCTTTTTCGGTGTTCTT	TGGGGTCGGTTTAATTTGCA	45.0	45.0	57.9	58.0	2843-2927 bp	0.833333	76.5
ndhF_p3	AGGCCGAATCTGCACAAAAT	TGGGGTCGGTTTAATTTGCA	45.0	45.0	58.5	58.0	2701-2747 bp	0.833333	71.9
ndhF_p4	TGGCTTGGAGCACTAGTACT	TAACCGGTTAAATGGGGTCG	50.0	50.0	58.1	57.6	2919-2980 bp	0.75	69.2
ndhF_p5	AGGAAAGAAGGCCGAATCTG	TGGGGTCGGTTTAATTTGCA	50.0	45.0	57.6	58.0	2709-2733 bp	0.75	68.5