Hotspot

ndhD

SSC · 121032-122534 · 1503 bp

Back to Vincetoxicum

Pi 0.122
MarkerSeek score 80.80
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1503
variable_sites
602
pi
0.122
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 ATTCGGCAATTGTAGCGTCT CAAATTGCTTCGGCTCCAAG 45.0 50.0 58.0 58.0 2127-2135 bp 0.833333 74.6
ndhD_p2 ATTCGGCAATTGTAGCGTCT AAGATGTGAATCCGCCTGTC 45.0 50.0 58.0 58.0 2083-2091 bp 0.833333 74.5
ndhD_p3 ATTCGGCAATTGTAGCGTCT AACAGAAGACTGCGTTGGTT 45.0 45.0 58.0 58.0 2107-2115 bp 0.833333 74.5
ndhD_p4 CTAACGAAGCTTGGGGATCC CAAATTGCTTCGGCTCCAAG 55.0 50.0 58.0 58.0 2247-2255 bp 0.833333 74.4
ndhD_p5 AGTCGTTTTTCGTTGGGGAA CAAATTGCTTCGGCTCCAAG 45.0 50.0 58.0 58.0 2437-2444 bp 0.75 70.7