Hotspot

ndhA

SSC · 125220-127373 · 2154 bp

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Pi 0.127
MarkerSeek score 82.80
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2154
variable_sites
891
pi
0.127
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 AGACAGGGTAATCGCGAAAC TCCAACCTTTGAATTGGCGA 50.0 45.0 58.0 58.0 2558-2587 bp 0.833333 74.8
ndhA_p2 AGACAGGGTAATCGCGAAAC GTGAGAGTTGAAGCCCCAAA 50.0 50.0 58.0 58.0 2524-2553 bp 0.833333 74.8
ndhA_p3 AGACAGGGTAATCGCGAAAC TACAACAGGCTCTGGAAGGA 50.0 50.0 58.0 58.0 2682-2711 bp 0.833333 74.7
ndhA_p4 AGACAGGGTAATCGCGAAAC GTTTATCGGGCCCAATGCTA 50.0 50.0 58.0 58.0 2853-2882 bp 0.833333 74.6
ndhA_p5 AGACAGGGTAATCGCGAAAC TTTATCGGGCCCAATGCTAC 50.0 50.0 58.0 58.0 2852-2881 bp 0.833333 74.6