Hotspot

rps15-ycf1

SSC · 128928-129306 · 379 bp

Back to Vincetoxicum

Pi 0.117

MarkerSeek score 69.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 379
variable_sites: 146
pi: 0.117
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACGCGTTACATAGGGCAAAT	TCTGCATCCGCGATTGTAAA	45.0	45.0	58.0	58.0	1508-1524 bp	0.833333	75.2
rps15-ycf1_p2	ACGCGTTACATAGGGCAAAT	TTCTGCATCCGCGATTGTAA	45.0	45.0	58.0	58.0	1509-1525 bp	0.833333	75.2
rps15-ycf1_p3	ACGCGTTACATAGGGCAAAT	TTTCTGCATCCGCGATTGTA	45.0	45.0	58.0	58.0	1510-1526 bp	0.833333	75.1
rps15-ycf1_p4	AACACCATGCATTGATGGGT	TCTGCATCCGCGATTGTAAA	45.0	45.0	58.1	58.0	1376-1392 bp	0.833333	75.0
rps15-ycf1_p5	ACGCGTTACATAGGGCAAAT	TTGGACTGAGAACAACTGCC	45.0	50.0	58.0	58.0	1173-1189 bp	0.583333	64.8