Hotspot

ndhG-ndhI

SSC · 124102-124637 · 536 bp

Back to Vincetoxicum

Pi 0.139

MarkerSeek score 77.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 536
variable_sites: 229
pi: 0.139
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACCTCCTAAGATCAGACCCG	GTTTCGCGATTACCCTGTCT	55.0	50.0	57.9	58.0	990-1053 bp	0.833333	79.4
ndhG-ndhI_p2	CGCGGCAGCTACAAAATAAG	GTTTCGCGATTACCCTGTCT	50.0	50.0	57.9	58.0	1104-1167 bp	0.833333	78.8
ndhG-ndhI_p3	ACAGTCCAAAGGCGAGAATC	GTTTCGCGATTACCCTGTCT	50.0	50.0	57.9	58.0	1205-1268 bp	0.833333	78.2
ndhG-ndhI_p4	TCCAAAGGCGAGAATCCTTG	GTTTCGCGATTACCCTGTCT	50.0	50.0	57.9	58.0	1201-1264 bp	0.833333	78.1
ndhG-ndhI_p5	GTCCAAAGGCGAGAATCCTT	GTTTCGCGATTACCCTGTCT	50.0	50.0	57.9	58.0	1202-1265 bp	0.833333	78.1