Hotspot

ccsA-ndhD

SSC · 120786-121031 · 246 bp

Back to Vincetoxicum

Pi 0.121

MarkerSeek score 70.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 246
variable_sites: 97
pi: 0.121
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCGGCAATTGTAGCGTCT	ATGAGTGGCTTTGTTGCTGA	45.0	45.0	58.0	58.0	692-695 bp	0.833333	74.9
ccsA-ndhD_p2	CTAACGAAGCTTGGGGATCC	ATGAGTGGCTTTGTTGCTGA	55.0	45.0	58.0	58.0	812-815 bp	0.833333	74.4
ccsA-ndhD_p3	AGTCGTTTTTCGTTGGGGAA	ATGAGTGGCTTTGTTGCTGA	45.0	45.0	58.0	58.0	1001-1004 bp	0.75	70.8
ccsA-ndhD_p4	ATTCGGCAATTGTAGCGTCT	TATCTTGACGAAATGGGCGG	45.0	50.0	58.0	58.1	787-788 bp	0.75	70.3
ccsA-ndhD_p5	AGTCGTTTTTCGTTGGGGAA	TATCTTGACGAAATGGGCGG	45.0	50.0	58.0	58.1	1096-1097 bp	0.666667	66.5