Hotspot

rpl32-trnL-UAG

SSC · 118759-119654 · 896 bp

Back to Vincetoxicum

Pi 0.143

MarkerSeek score 81.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 896
variable_sites: 406
pi: 0.143
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGCAAATTAAACCGACCCCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1397-1621 bp	0.833333	79.1
rpl32-trnL-UAG_p2	TGCAAATTAAACCGACCCCA	AGCAAAGCCCTGGGTAAAAA	45.0	45.0	58.0	57.9	1861-2087 bp	0.833333	78.7
rpl32-trnL-UAG_p3	TGCAAATTAAACCGACCCCA	AACCACTCGGATTGAAGAGC	45.0	50.0	58.0	57.9	1929-2155 bp	0.833333	78.6
rpl32-trnL-UAG_p4	TGCAAATTAAACCGACCCCA	TTCCCCAACGAAAAACGACT	45.0	45.0	58.0	58.0	2083-2309 bp	0.75	75.3
rpl32-trnL-UAG_p5	CTCCCCCTCCTTAGCTTTCT	TTCCCCAACGAAAAACGACT	55.0	45.0	58.1	58.0	1930 bp	0.083333	42.1