Hotspot

ndhF-rpl32

SSC · 117809-118599 · 791 bp

Back to Vincetoxicum

Pi 0.119

MarkerSeek score 83.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 791
variable_sites: 298
pi: 0.119
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTTGAGGTCGTGTGAACCAA	GTTCGAAACCTCTCCCCATC	50.0	55.0	58.1	58.0	1644-1881 bp	0.75	75.4
ndhF-rpl32_p2	CTTGAGGTCGTGTGAACCAA	TCGAAACCTCTCCCCATCTT	50.0	50.0	58.1	58.0	1642-1879 bp	0.75	75.2
ndhF-rpl32_p3	CTTGAGGTCGTGTGAACCAA	TTCGAAACCTCTCCCCATCT	50.0	50.0	58.1	58.0	1643-1880 bp	0.75	75.2
ndhF-rpl32_p4	GGCACCACCAAATACCAGAA	GTTCGAAACCTCTCCCCATC	50.0	55.0	58.1	58.0	1844-2081 bp	0.666667	71.5
ndhF-rpl32_p5	GGCACCACCAAATACCAGAA	TCGAAACCTCTCCCCATCTT	50.0	50.0	58.1	58.0	1842-2079 bp	0.666667	71.3