Hotspot

trnE-UUC-trnT-GGU

LSC · 35846-36723 · 878 bp

Back to Vincetoxicum

Pi 0.00609

MarkerSeek score 69.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 22
pi: 0.00609
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	949-1053 bp	1.0	84.3
trnE-UUC-trnT-GGU_p2	ACTTCCTTTCCTAGTCCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1011-1115 bp	0.916667	81.3
trnE-UUC-trnT-GGU_p3	CTTCCTTTCCTAGTCCCCCA	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1010-1114 bp	0.916667	81.3
trnE-UUC-trnT-GGU_p4	ACTTCCTTTCCTAGTCCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.0	57.9	976-1080 bp	0.916667	80.7
trnE-UUC-trnT-GGU_p5	CTTCCTTTCCTAGTCCCCCA	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.0	57.9	975-1079 bp	0.916667	80.7