Hotspot

ycf1

IRb · 109347-110384 · 1038 bp

Pi 0.0183

MarkerSeek score 54.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1038
variable_sites: 19
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	TCCTTTCAATCAAGCGGGAG	55.0	50.0	58.0	57.9	1940-1971 bp	1.0	80.5
ycf1_p2	CAATTGTGACCTCTCGGGAG	TCCTTTCAATCAAGCGGGAG	55.0	50.0	58.0	57.9	2276-2307 bp	1.0	80.3
ycf1_p3	GCGAGCCCCTTTATTCTGAA	TCCTTTCAATCAAGCGGGAG	50.0	50.0	58.0	57.9	2050-2081 bp	1.0	80.2
ycf1_p4	AAGCGAGCCCCTTTATTCTG	TCCTTTCAATCAAGCGGGAG	50.0	50.0	58.0	57.9	2052-2083 bp	1.0	80.1
ycf1_p5	TTGTGACCTCTCGGGAGAAT	TCCTTTCAATCAAGCGGGAG	50.0	50.0	58.1	57.9	2273-2304 bp	1.0	79.9