Hotspot

ndhD

SSC · 115335-116486 · 1152 bp

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Pi 0.00434
MarkerSeek score 46.20
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1152
variable_sites
5
pi
0.00434
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 CCATCCGGGGTTCAAATTCA GATACCCTGGGACGGATGTA 50.0 55.0 58.2 58.0 1915-1916 bp 1.0 79.6
ndhD_p2 CCATCCGGGGTTCAAATTCA AGCTACTTTATCGGCTTGGC 50.0 50.0 58.2 58.0 1337 bp 1.0 79.4
ndhD_p3 GGTGCAAATTCGGCAATTGT GATACCCTGGGACGGATGTA 45.0 55.0 58.2 58.0 2150-2151 bp 1.0 79.4
ndhD_p4 TGCAAATTCGGCAATTGTGG GATACCCTGGGACGGATGTA 45.0 55.0 58.2 58.0 2148-2149 bp 1.0 79.3
ndhD_p5 CCATCCGGGGTTCAAATTCA TTCGGGTTTATTTAGGGGCG 50.0 50.0 58.2 58.0 1805 bp 0.5 58.4