Hotspot

trnL-CAA-ycf15

IRa · 143675-144075 · 401 bp

Back to Vinca

Pi 0.015
MarkerSeek score 63.20
Primer available yes
Label trnL-CAA-ycf15

Feature Metadata

label_name
trnL-CAA-ycf15
feature_type
igs
region
IRa
length_bp
401
variable_sites
6
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf15_p1 AAATGGTAGACACGCGAGAC TGAACAGCGAACAACCAGTT 50.0 45.0 58.0 58.3 538 bp 1.0 78.8
trnL-CAA-ycf15_p2 TGAAATGGTAGACACGCGAG TGAACAGCGAACAACCAGTT 50.0 45.0 58.0 58.3 540 bp 1.0 78.8
trnL-CAA-ycf15_p3 GCCTTGGTGGTGAAATGGTA TGAACAGCGAACAACCAGTT 50.0 45.0 58.1 58.3 550 bp 1.0 78.2
trnL-CAA-ycf15_p4 TGGTGAAATGGTAGACACGC TGAACAGCGAACAACCAGTT 50.0 45.0 58.2 58.3 543 bp 1.0 77.3
trnL-CAA-ycf15_p5 GAAATGGTAGACACGCGAGA TGAACAGCGAACAACCAGTT 50.0 45.0 57.7 58.3 539 bp 1.0 76.7