Hotspot

petA-psbJ

LSC · 63544-64621 · 1078 bp

Back to Vinca

Pi 0.0205
MarkerSeek score 60.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1078
variable_sites
22
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1134-1137 bp 1.0 77.4
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1136-1139 bp 1.0 77.4
petA-psbJ_p3 AGAAGGTTCAATTGGCCGAA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.7 57.6 1133-1136 bp 1.0 76.6
petA-psbJ_p4 GGTTCAATTGGCCGAAATGA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.0 57.6 1129-1132 bp 1.0 70.6
petA-psbJ_p5 GAGAAGGTTCAATTGGCCGA TGGTTCATATTCCGGATTGGG 50.0 47.6 58.2 57.8 1142-1145 bp 1.0 70.5